Biotin Quantitation Kit

Biotin Quantitation Kit

Catalog	BR00028226
Description	The Biotin Quantitation Kit allows for the rapid determination of the molar ratio of biotin to protein.
Application	This kit is mainly used to label primary antibodies, secondary antibodies, soluble MHC/peptide complexes (monomers), recombinant proteins. In biology, biotin-labeled molecules are used for: FC, ELISA Detection, ELISA, WB, IHC-P, ICFC.
Storage	Store the kit unopened at 2-8℃ for 1 year, and the dissolved biotin can be stored at 2-8℃ for 1 week.
Component	HABA-Avidin premix 18 vials
Feature	To measure biotin incorporation, a solution containing the biotinylated protein is mixed with HABA and avidin. Since biotin has a higher affinity for avidin, it displaces HABA from the avidin complex, leading to a proportional decrease in absorbance at 500 nm. This method enables the quantification of biotin in a solution by measuring the absorbance of the HABA-avidin mixture before and after adding the biotin-containing sample in a single cuvette. The change in absorbance corresponds to the amount of biotin present.

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